Research Paper Volume 12, Issue 2 pp 1304—1321

AURKB promotes gastric cancer progression via activation of CCND1 expression

Figure 1. AURKB knockdown inhibits gastric cancer cell proliferation. (A), Quantitative real-time PCR analysis of the effect of AURKB knockdown by siRNA on the mRNA levels of AURKB in SGC7901 and BGC823 cells. The results shown are the means ± SDs of three independent experiments; **, P < 0.01 compared with the scrambled negative control (NC). (B) Western blot analysis of AURKB and H3S10ph expression in siRNA- and NC-transfected SGC7901 and BGC823 cells. HSP70 and histone H3 were used as loading controls. (C) CCK-8 assays showing that AURKB knockdown by siRNA significantly inhibited the proliferation of SGC7901 and BGC823 cells. The results shown are the means ± SDs of three independent experiments; **, P < 0.01 compared with the negative control. (D) Effects of AURKB knockdown by siRNA on the colony formation ability of SGC7901 and BGC823 cells. Left panel, representative images from colony formation assays. Right panel, the number of colonies formed by the indicated cells was quantified. Data are presented as the means ± SDs; **, P<0.01 compared with the negative control. (E) Flow cytometry analysis showing the cell cycle distribution of SGC7901 and BGC823 cells transfected with negative control (NC) or AURKB siRNA. Bar graphs showing the percentages of SGC7901 and BGC823 cells in the G0/G1, S and G2/M phases when treated with negative control (NC) or AURKB siRNAs (right panel). Each histogram bar represents the mean ± SD of three independent experiments.