Research Paper Volume 12, Issue 2 pp 1397—1416

Activated Drp1-mediated mitochondrial ROS influence the gut microbiome and intestinal barrier after hemorrhagic shock

Figure 1. The activation and mitochondrial translocation of Drp1 in IECs after shock or hypoxia. (A) The mitochondrial translocation of Drp1 after hemorrhagic shock in colon tissues. N_WT, WT mice in normal condition; HS_WT, WT mice in hemorrhagic shock condition. (n=5 mice/ group) (B) Post-translational modifications of Drp1 after hemorrhagic shock detected by co-immunoprecipitation. N, N_WT group; HS, HS_WT group; Phosphor-, Phosphorylation; Ubiquitin-, ubiquitination; SUMO-, SUMOylation. (n=5 mice/ group) (C) The phosphorylation of Drp1 after hemorrhagic shock in colon tissues detected by Western Blotting. (n=5 mice/ group) (D) The co-location of Drp1 and mitochondria after CoCl2-induced hypoxia in IECs. (Bar, 25μm) (n=5). a represents p < 0.05 compared with N_WT group or Normal group.