Research Paper Volume 12, Issue 2 pp 1397—1416

Activated Drp1-mediated mitochondrial ROS influence the gut microbiome and intestinal barrier after hemorrhagic shock

Figure 4. The effects of Drp1 on gut microbiome composition and SCFA production after shock. (A) The relative abundance of gut microbiome composition in each group detected by 16S rRNA gene sequencing (8 mice/ group). (B) The ratio of Bacteroidetes/Firmicutes in each group (8 mice/ group). (C) Relative Bacteroidetes abundance in each group detected by metagenomics profiling (8 mice/ group). (D) Differentially-expressed gut microbiome classification after shock detected by phylogenetic tree. The levels include phylum, class, order, family and genus. Each abundance value is labeled under branches. (E) The contents of intestinal SCFAs, including acetic acid, propionic acid and butyric acid, in each group (n=8 mice/ group). N_WT, WT mice in normal condition; HS_WT, WT mice in hemorrhagic shock condition; HS_Drp1 KO, Drp1 KO mice in hemorrhagic shock condition. a represents p < 0.05 compared with N_WT group; b represents p < 0.05 compared with HS_WT group.