Figure 3. SRV2 triggers mitochondrial fission in cardiomyocytes. (A–C) Average mitochondrial length was measured and proportion of fragmented mitochondria was evaluated in an immunofluorescence assay after cardiomyocytes were transfected with siRNA against SRV2. (D–F) RNA was isolated from LPS-treated cardiomyocytes and qPCR was performed to analyze Drp1, Fis1, and Mff transcript levels. (G–H) RNA was isolated from LPS-treated cardiomyocytes and qPCR was performed to analyze Mfn2 and Opa1 transcript levels. *p<0.05 vs. control group, #p<0.05 vs. LPS group.