Figure 6. Cardiomyocyte mitochondrial metabolism is disrupted by SRV2-induced mitochondrial fission. (A) ATP production was measured in cardiomyocytes after transfection of siRNA against SRV2 and addition of FCCP to the culture medium. (B–D) RNA was isolated from LPS-treated cardiomyocytes and qPCR was performed to analyze ETC-1/2/3 transcript levels after SRV2 siRNA transfection and FCCP treatment. (E–G) Cardiomyocyte ETC-1/2/3 activities were measured via ELISA after SRV2 siRNA transfection and FCCP treatment. (H–I) The JC-1 probe was used to evaluate mitochondrial membrane potential in cardiomyocytes after SRV2 siRNA transfection and FCCP treatment; red-to-green fluorescence ratios indicate alterations in mitochondrial membrane potential. *p<0.05 vs. control group, #p<0.05 vs. LPS group, @p<0.05 vs. LPS+si-SRV2 group.