Research Paper Volume 12, Issue 2 pp 1417—1432

SRV2 promotes mitochondrial fission and Mst1-Drp1 signaling in LPS-induced septic cardiomyopathy

Figure 7. SRV2 promotes mitochondrial fission by activating the Mst1-Drp1 signaling pathway. (AB) RNA was isolated from LPS-treated cardiomyocytes and qPCR was performed to analyze Mst1 and Drp1 transcript levels after transfection of Mst1 overexpression adenovirus following SRV2-knockdown. (CD) Cardiomyocyte Mff and Fis1 transcript levels were measured through qPCR after Mst1 overexpression and SRV2 knockdown. (E) ATP production was measured in cardiomyocytes after Mst1 overexpression and SRV2 knockdown. (FH) Cardiomyocyte SOD, GSH, and GPX levels were measured via ELISA after transfection of SRV2 siRNA and Mst1 overexpression adenovirus. (IJ) ROS production was measured using ROS probe (K) and caspase-9 activity was determined via ELISA in cardiomyocytes after transfection of SRV2 siRNA and Mst1 overexpression adenovirus. *p<0.05 vs. control group, #p<0.05 vs. LPS group, @p<0.05 vs. LPS+si-SRV2 group.