Research Paper Volume 12, Issue 2 pp 1465—1487

MTDH promotes metastasis of clear cell renal cell carcinoma by activating SND1-mediated ERK signaling and epithelial-mesenchymal transition

Figure 2. Enhanced MTDH protein expression was associated with poor prognosis of ccRCC. (A) The representative immunostaining of MTDH in normal kidney and ccRCC, (Scale bar, 200 μm). (B) Immunohistochemistry staining analysis of MTDH protein expression in 111 RCC tissues and 61 adjacent normal kidney tissues. (C) The representative immunostaining of high and low MTDH expression in ccRCC tissues was shown. (D) The high MTDH mRNA expression group had a poorer OS than the low MTDH group in TCGA-KIRC dataset based on The Human Protein Atlas website. (E) In 111 ccRCC patients from Peking University First Hospital, compared to those with lower expression levels, ccRCC patients with high MTDH protein expression levels had shorter OS. (F) In 111 ccRCC patients from Peking University First Hospital, compared to those with lower expression levels, ccRCC patients with high MTDH protein expression levels had shorter CSS. (G) In 111 ccRCC patients from Peking University First Hospital, compared to those with lower expression levels, ccRCC patients with high MTDH protein expression levels had shorter PFS. (H) In 111 ccRCC patients from Peking University First Hospital, compared to those with lower expression levels, ccRCC patients with high MTDH protein expression levels had shorter MFS. (I) MTDH mRNA expression in metastasis ccRCC is higher than the MTDH mRNA expression in normal kidney tissues and primary ccRCC in GSE105288 dataset. (J) Compared to those without postsurgical metastasis, ccRCC patients with postsurgical metastasis had higher MTDH protein expression levels in PKU-KIRC dataset.