Research Paper Volume 12, Issue 2 pp 1465—1487

MTDH promotes metastasis of clear cell renal cell carcinoma by activating SND1-mediated ERK signaling and epithelial-mesenchymal transition

Figure 5. MTDH promotes metastasis largely by enhancing SND1-mediated EMT and ERK signaling. (A) SND1 mRNA expression was increased in ccRCC tissues in both TCGA dataset and GSE105288, especially in metastatic ccRCC. (B) Compared to those without postsurgical metastasis, ccRCC patients with postsurgical metastasis had higher SND1 protein expression levels in PKU-KIRC dataset. (C) The ccRCC patients with high SND1 protein expression levels had shorter MFS than those with lower SND1 expression levels (p = 0.0061). (D) Migration and invasive capabilities were attenuated in 786-O-SND1-knockdown cells(20x). (E) Silencing SND1 reduced the protein expression of p-ERK1/2, Snail in 786-O cells. (F) knockdown of SND1 could abolish promoting migration and invasive capabilities of ccRCC cells induced by overexpressing MTDH(20x). (G) Silencing SND1 could attenuate increased expression of snail and p-ERK protein induced by overexpressing MTDH.