Figure 6. Overexpression of AQP9 was able to suppress Wnt/β-catenin signaling. (A–D) The mRNA and protein levels of DVL2, GSK-3β, CyclinD1 and β-catenin in Huh-7 and SMMC-7721 cells transfected with LV-NC or LV-AQP9 were examined by RT-qPCR and western blotting. (E) The expression of β-catenin in HCC cells treated with XAV939 were evaluated using RT-qPCR. (F) The mRNA levels of β-catenin in SMMC-7721 cells were determined following the treatment with SKL2001. (G) The protein levels of β-catenin in HCC cells were assessed by western blot analysis. The results were represented as mean ± SD. P<0.05 vs. NC or LV-NC. Each experiment was repeated 3 times. AQP, aquaporin 9; HCC, hepatocellular carcinoma; NC, negative control; RT-qPCR, reverse transcriptase-quantitative polymerase chain reaction.