Research Paper Volume 12, Issue 2 pp 1527—1544

Aquaporin 9 inhibits growth and metastasis of hepatocellular carcinoma cells via Wnt/β-catenin pathway

Figure 7. Blockage of Wnt/β-catenin signaling inhibited growth and metastasis of HCC cells. (A) The proliferation of Huh-7 cells treated with XAV939 was determined by CCK-8 assay. (B) The mRNA levels of E-cad, N-cad and α-SMA in transfected HCC cells were examined using RT-qPCR. (C and D) The expression of PCNA in Huh-7 cells were evaluated following the treatment with XAV939. (E to H) The invasive and migrative abilities of HCC cells treated with XAV939 were assessed by Transwell assay (magnificationx100). (I and J) Cell apoptosis following the treatment with XAV939 was determined using flow cytometry. The results were represented as mean ± SD. P<0.05 vs. NC. Each experiment was repeated 3 times. HCC, hepatocellular carcinoma; RT-qPCR, reverse transcriptase-quantitative polymerase chain reaction.