Figure 9. Overexpression of AQP9 suppressed tumor growth of HCC in vivo. (A) The growth curves of tumors from nude mice in LV-NC and LV-AQP9 group. (B and C). Orthotopic tumor volumes and weights at day 42 post-injection were calculated. (D) The protein levels of AQP9, DVL2, GSK-3β, CyclinD1 and β-catenin in isolated tumors were examined using western blotting. (E) The mRNA levels of E-cad, N-cad and α-SMA were determined by RT-qPCR. The results were represented as mean ± SD. P<0.05 vs. LV-NC. AQP, aquaporin 9; HCC, hepatocellular carcinoma; NC, negative control; RT-qPCR, reverse transcriptase-quantitative polymerase chain reaction.