Research Paper Volume 12, Issue 2 pp 1591—1609

Chikusetsu saponin IVa protects pancreatic β cell against intermittent high glucose-induced injury by activating Wnt/β-catenin/TCF7L2 pathway

Figure 1. CHS protected against proliferation and cytotoxicity of islet cells from IHG. (A) The chemical structure of CHS. Molecular weight: 794. Molecular formula: C42H66O14. The glucose stimulated insulin secretion in primary pancreatic islet cells (B) and βTC3 cells (C) were measured by an insulin RIA kit after incubation for 24, 48 and 72 h. The insulin secretion levels in primary pancreatic islet cells (D) and βTC3 cells (E) in response to 3.0 mM and 27.8 mM glucose stimulation. Cell viability of primary pancreatic islet cells (F) and βTC3 cells (G) was measured by a CCK-8 assay. Cytotoxicity in primary pancreatic islet cells (H) and βTC3 cells (I) was measured by an LDH assay. Data are representative of three independent experiments. ##P<0.01 vs. NG treatment group, **P<0.01 vs. SHG treatment group, ΔΔP<0.01 vs. IHG treatment group.