Figure 2. CHS protected against apoptosis of islet cells from IHG. (A) The apoptosis rate of βTC3 cells was assessed using flow cytometry with Annexin-V/PI staining. (B) The cell nuclear morphology of βTC3 cells was measured by DAPI staining. Fluorescence microscope (200×) images. (C) The apoptosis related proteins expression levels were measured by western blotting according to the methods 2.12. Data are representative of three independent experiments. ##P<0.01 vs. NG treatment group, **P<0.01 vs. SHG treatment group, ΔΔP<0.01 vs. IHG treatment group.