Figure 3. CHS protected against cell cycle arrest of islet cells from IHG. (A) The βTC3 cell proliferation was measured by a BrdU labeling and detection kit. Inverted microscope (200×) images. (B) The cell cycle related proteins (Cyclin D1, Skp2, p21, andp27) were measured by western bolting. Data are representative of three independent experiments. ##P<0.01 vs. NG treatment group, **P<0.01 vs. SHG treatment group, ΔΔP<0.01 vs. IHG treatment group.