Research Paper Volume 12, Issue 2 pp 1591—1609

Chikusetsu saponin IVa protects pancreatic β cell against intermittent high glucose-induced injury by activating Wnt/β-catenin/TCF7L2 pathway

Figure 5. Effects of CHS might through TCF7L2 pathway. (A) The protein expression of TCF7L2 after siRNA transfection target TCF7L2. (B) The protein expression levels of TCF7L2 downstream proteins after inhibition of TCF7L2 by TCF7L2 siRNA. (C) The apoptosis rate of βTC3 cell was measured by flow cytometry after inhibition of TCF7L2 by TCF7L2 siRNA. (D) The BrdU immunohistochemical staining was performed after inhibition of TCF7L2 by TCF7L2 siRNA. (E) The effects of siTCF7L2 on the glucose induced insulin secretion. (F) The effects of siTCF7L2 on the GLP-1 induced insulin secretion. Data are representative of three independent experiments. ##P<0.01 vs. NG treatment group, **P<0.01 vs. IHG treatment group, ΔΔP<0.01 vs. scrb treatment group.