Research Paper Volume 12, Issue 2 pp 1591—1609

Chikusetsu saponin IVa protects pancreatic β cell against intermittent high glucose-induced injury by activating Wnt/β-catenin/TCF7L2 pathway

Figure 7. Effects of CHS on HBP1 expression in islets β cell. (A) Time dependent effects of CHS on the expression HBP1. (B) Dose dependent effects of CHS on the expression HBP1. **P<0.01 vs. 0h or 0mM treatment group. (C) Effects of CHS on HBP1 expression after IHG or SHG treatment. βTC3 cells were transfected with the empty vector pcDNA3 (scrb) or vectors encoding HA-tagged wild-type HBP1 (HBP1) at 30nM, and then cells were exposed to different treatments as indicated. (D) The protein expression levels of TCF7L2, GIPR, cyclinD1, Skp2, p53 and p21 were measured by western blotting. (E) Insulin secretion levels was measured by an insulin RIA kit. (F) Cell variability was measured by CCK8 assay. (G) BrdU positive ratio was calculated from the BrdU positive cell numbers vs total cell numbers. βTC3 cells were transfected with scrb and siHBP1 (30nM) for 24h, and then cells were exposed to different treatments as indicated. (H) The protein expression levels of GIPR, cyclinD1, and p53 were measured by western blotting. (I) Cell variability was measured by CCK8 assay. (J) Insulin secretion levels was measured by an insulin RIA kit. (K) BrdU positive ratio was calculated from the BrdU positive cell numbers vs total cell numbers. Data are representative of three independent experiments. ##P<0.01 vs. NG treatment group, ΔΔP<0.01 vs. IHG treatment group, &&P<0.01 vs.scrb treatment group.