Figure 2. IL-33 promotes glioma cell invasion, migration and mesenchymal transition. (A) Effects of IL-33 on glioma cells invasion. Glioma cell lines U251 and Ln229 were subject to transwell assay for 24 hours. IL-33 was added in different concentrations. (B, C) The number of cells moved through the chambers were counted for per field of view and analyzed. Each column represents three independent experiments; Results are expressed as the mean±SD; n=3; ***, P < 0.001; ****, P < 0.0001. (D–E) IL-33 promotes the migration of glioma cells U251 by wound healing assay. The tumor cells moving distance was detected and divided by control group as relative migration distance for statistical analysis. Results are expressed as the mean±SD; n=3; ****, P < 0.0001. (F) The levels of core epithelial marker E-cadherin and mesenchymal markers including vimentin, N-cadherin and β-catenin were detected by Western blotting.