Figure 3. IL-33 promotes glioma stemness. (A) Effects of IL-33 on glioma cells stemness. Glioma cell lines U251 and Ln229 were subject to sphere formation assay for 7 days. IL-33 was added in different concentrations. Representative images of spheres of glioma cells are shown. Scale bar, 200 μm. (B and C) The mean numbers and diameters of spheres were counted and analyzed. Each column represents three independent experiments; Results are expressed as the mean±SD; n=3; *, P < 0.05; **, P < 0.01. (D and E) The levels of core stem cell genes including CD133, Nestin, Sox2 and Oct4 were detected by Western blot.