Research Paper Volume 12, Issue 2 pp 1725—1746

Female adipose tissue has improved adaptability and metabolic health compared to males in aged obesity

Figure 5. Oxidative metabolism and ECM remodeling is highly active in older obese female mice. Relative expression of GWAT oxidative genes - (A) Adrb3 (B) Pparγ (C) Pgc1α. Expression of GWAT senescence genes (D) p53 (E) p16 (F) p21. Expression of GWAT fibrosis and ECM remodeling genes - (G) Mmp2 (H) Mmp9 (I) Timp1 (J) Tgfβ1 (K) α-sma (L) Col1a1 in young and old obese male and female GWAT. A.U., arbitrary units normalized to Gapdh. N=5-8. (M) Collagen content in GWAT of young and old obese male and female mice, N=5. (N) Picrosirius red staining of young and old, male and female HFD GWAT positive area as a percentage. N=5. Two-way ANOVA with Bonferroni-Dunn’s post-test was performed for (AL). Statistics from diet and sex interaction are in box. Statistical significance is indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Student’s t-test was performed for male and female comparisons between the same diet groups indicated by #p<0.05, ##p<0.01 ###p<0.001 and ####p<0.0001; error bars are SEM. One-way ANOVA with Student’s t-test was performed for (MN). Statistical significance is indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; error bars are SEM.