Research Paper Volume 12, Issue 2 pp 1747—1759

K63 ubiquitin chains target NLRP3 inflammasome for autophagic degradation in ox-LDL-stimulated THP-1 macrophages

Figure 2. Ox-LDLs inhibit autophagy in a time- and dose-dependent manner. (A) The immunoblot analysis of lysates of Mφ treated with ox-LDLs (50 ug/ml) for a series of time intervals. (B) The immunoblot analysis of lysates of Mφ treated with various doses of ox-LDLs for 24 hours. (A and B) The densitometric analysis of the p62 signal and LC3II/LC3I ratio vs. time (A) and ox-LDL concentrations (B), which was normalized to β-actin. The data are presented as mean ± SD (n=3).