Research Paper Volume 12, Issue 2 pp 1747—1759

K63 ubiquitin chains target NLRP3 inflammasome for autophagic degradation in ox-LDL-stimulated THP-1 macrophages

Figure 5. The main target of p62 is more likely to be NLRP3. (A above) The immunoblot analysis of the NLRP3, ASC and p62 immunoprecipitates of cell lysates obtained from Mφ, which had been stimulated with ox-LDLs (50 ug/ml) for 24 hours. (A below) The densitometric analysis of the NLRP3, ASC and p62 signal, which were normalized to the corresponding signals in the p62 immunoprecipitates. (B) The densitometric analysis of the ratios of NLRP3/ASC obtained from the NLRP3 immunoprecipitates and the ASC immunoprecipitates. (C) The densitometric analysis of p62 signal detected in the NLRP3 immunoprecipitates and the ASC immunoprecipitates, and normalized to the corresponding NLRP3 and ASC signal respectively. The data are presented as mean ± SD (n=3); * denotes the statistical significance by one-way ANOVA with post hoc Dunnett’s multiple comparisons test (A) or by t-test (B and C). *P<0.05, **P<0.01, ***P<0.001.