Research Paper Volume 12, Issue 2 pp 1760—1777

Nesfatin-1 suppresses interleukin-1β-induced inflammation, apoptosis, and cartilage matrix destruction in chondrocytes and ameliorates osteoarthritis in rats

Figure 10. Effect of nesfatin-1 on the IL-1β-induced NF-κB and MAPK activation in rat chondrocytes. (A, C). Chondrocytes were treated with different concentrations of nesfatin-1 for 6 h after IL-1β-induction. The protein levels of p38, phosphor-p38, JNK, phosphor-JNK, p65, and phosphor-p65 were evaluated via western blot analysis (B, D, and E). Quantitative analysis of western blot in rat chondrocytes. The values are expressed as mean ±SD (n=3) and were analyzed by one-way analysis of variance followed by Tukey's post hoc test.*p < 0.05, **p < 0.01 versus the IL-1β group.