Figure 2. Effect of nesfatin-1 and IL-1β on cell viability. (A) Cells treated with increasing concentrations of nesfatin-1 (0, 0.1, 1 to 1000 ng/mL) for 24 h. (B) Pre-treatment with nesfatin-1 for 2 hours, followed by IL-1β treatment for 24 h. Chondrocyte viability was evaluated via CCK-8 analysis. Data represent the mean ± SD (n=3) and were analyzed by one-way analysis of variance followed by Tukey's post hoc test.* p < 0.05 versus IL-1β-treated alone samples.