Figure 3. Apoptosis rate determination using flow cytometry of nesfatin-1- and IL-1β-treated cells. (A) Cells pre-treated with nesfatin-1 (10 ng/mL) for 2 hours, followed by IL-1β treatment for 24 hours, and analyzed by flow cytometry. (B) Flow cytometric analysis. IL-1β-stimulation alone significantly increased the percentage of apoptotic chondrocytes. Annexin V- and PI-positive cells were markedly decreased among nesfatin-1 pre-treated cells. Data represent the mean ± SD (n=3) and were analyzed by one-way analysis of variance followed by Tukey's post hoc test. ** p < 0.01 versus the IL-1β group.