Research Paper Volume 12, Issue 2 pp 1760—1777

Nesfatin-1 suppresses interleukin-1β-induced inflammation, apoptosis, and cartilage matrix destruction in chondrocytes and ameliorates osteoarthritis in rats

Figure 4. Effect of nesfatin-1 on IL-1β-induced activation of apoptosis in rat chondrocytes. (A) Chondrocytes were treated with nesfatin-1 on IL-1β-induced chondrocytes for 6 h and the protein levels of Bcl-2, Bax, cleaved caspase-3, pro-caspase-3, cleaved PARP, and PARP were evaluated via western blot analysis. (BD) Quantitative analysis of western blot in rat chondrocytes. Data represent the mean ± SD (n=3) and were analyzed by one-way analysis of variance followed by Tukey's post hoc test. ** p < 0.01 versus the IL-1β group.