Research Paper Volume 12, Issue 2 pp 1760—1777

Nesfatin-1 suppresses interleukin-1β-induced inflammation, apoptosis, and cartilage matrix destruction in chondrocytes and ameliorates osteoarthritis in rats

Figure 5. Nesfatin-1 suppressed the IL-1β-induced MMP-3, MMP-9, and MMP-13 expression in rat chondrocytes and the secretion level in the supernatant. Chondrocytes were treated with different concentrations of nesfatin-1 on IL-1β-induced chondrocytes for 24 h. The secretion level of MMPs was evaluated by ELISA (A, B, and C) and the mRNA level of MMPs was evaluated via real-time PCR (D, E, and F). The values are expressed as mean ±SD (n=3) and were analyzed by one-way analysis of variance followed by Tukey's post hoc test. **p < 0.01 versus the IL-1β group.