Research Paper Volume 12, Issue 2 pp 1760—1777

Nesfatin-1 suppresses interleukin-1β-induced inflammation, apoptosis, and cartilage matrix destruction in chondrocytes and ameliorates osteoarthritis in rats

Figure 9. Nesfatin-1 suppressed the IL-1β-induced IL-6, NO, and PGE2 expression in rat chondrocytes and the supernatant. Chondrocytes were treated with different concentrations of nesfatin-1 on IL-1β-induced chondrocytes for 24 h. The secretion level of IL-6 in the supernatant was evaluated via ELISA (A) and the mRNA level of IL-6 was evaluated via real-time PCR (B). The NO level was evaluated via Griess reaction (C) and the PGE2 level was evaluated via ELISA (D). The values are expressed as mean ±SD (n=3) and were analyzed by one-way analysis of variance followed by Tukey's post hoc test. *p<0.05, **p < 0.01 versus the IL-1β group.