Research Paper Volume 12, Issue 2 pp 1792—1807

The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors

Figure 1. Effects of an activator and inhibitor of SIRT1 on learning and memory, expression and activity of SIRT1, and the expression of α7 nAChR in mice carrying the APP/PS1 double mutation. The wild-type (WT) animals received physiological saline (PS) and APP/PS1 mice RSV (20 mg/kg) or suramin (20 mg/kg) by gavage once daily for two months. (A) Escape latency. (B) The numbers of platform crossings (N). (C) Time spent at the original position of platform. (D) Relative expression of SIRT1 protein in brain tissue, as determined by Western blotting. (E) SIRT1 activity in the brain tissue. (G) Correlation between the levels of SIRT1 and α7 nAChR in mice carrying the APP/PS1 double mutation and treated with RSV. (H) Correlation between the levels of SIRT1 and α7 nAChR in mice carrying the APP/PS1 double mutation and treated with suramin. The values presented are means ± SD. *P<0.05 compared with the WT group; #P<0.05 compared with the APP/PS1 group, as determined by analysis of variance (ANOVA), followed by the Tukey HSD test. Representative western blots are shown beneath D and F.