Figure 5. Effects of RSV and suramin on the expression of αAPP, α7 nAChR and SIRT1, as well as SIRT1 activating in SH-SY5Y/APPswe cells. The cells were treated with 50μM RSV or 300 μg/ml suramin for 24 h. (A) SIRT1 expression. (B) SIRT1 activity. (C) αAPP expression. (D) α7 nAChR expression. The values presented are means ± SD. *P<0.05 and **P<0.01 compared with untreated cells, #P<0.05 and ##P<0.01 compared to the untreated control cells, as determined by analysis of variance (ANOVA), followed by the Tukey HSD test. Representative western blots are shown beneath A, C and D.