Research Paper Volume 12, Issue 2 pp 1792—1807

The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors

Figure 7. SIRT1 regulates expression of α7 nAChR and p-ERK1/2 in SH-SY5Y cells through the MAPK pathway. (A) Effects of RSV and suramin on the level of α7 nAChR protein, as determined by Western blotting. (B) Effects of RSV and suramin on the level of ERK1/2, as determined by Western blotting. (C) Lack of effect of PNU or MLA on the level of SIRT1 protein. (D) Lack of effect of PNU or MLA on the level of ERK1/2 protein. (E) Lack of effect of U0126 on the level of SIRT1 protein. (F) Effect of U0126 on the level of α7 nAChR protein. The values presented are means ± SD. *P<0.05 and **P<0.01 compared to the SIRT1 siRNA negative control cells, #P<0.05 compared to treatment with U1026, as determined by analysis of variance (ANOVA), followed by the Tukey HSD test. Representative western blots are shown beneath each bar graph.