Figure 4. ISG20 was up-regulated in ccRCC. (A) The mRNA expression level of ISG20 was elevated in ccRCC tissues. (B–E) ISG20 was up-regulated in ccRCC tissues in the online databases (TCGA KIRC dataset, GSE40435 dataset, and Oncomine database). (F, G) Both mRNA and protein expression levels of ISG20 were elevated in ccRCC cell lines. (H, I) The protein expression level of ISG20 was higher in ccRCC tissues than that in normal tissues. ccRCC: clear cell renal cell carcinoma; TCGA KIRC: The Cancer Genome Atlas Kidney Clear Cell Carcinoma; qRT-PCR: quantitative real-time PCR; IHC: Immunohistochemistry. Data are represented as mean ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05.