**Figure 1.** **PSMA3-AS1 is overexpressed in ESCC tissues and higher PSMA3-AS1 expression is correlated with a poor prognosis for ESCC.** (**A**) PSMA3-AS1 expression in ESCC tissues and their corresponding adjacent non-tumor tissues according to RT-qPCR analysis. GAPDH was used as an internal control for loading. (**B**) In total, 120 patients were divided into groups with and without distant metastasis. The diagram shows PSMA3-AS1 expression in each group. ***p < 0.001. (**C**) In total, 120 patients were divided into ≤ 4 cm and > 4 cm size groups. The diagram shows PSMA3-AS1 expression in each group. ***p < 0.01. (**D**) and (**E**) The OS and cumulative recurrence rates of 120 patients with ESCC were compared between the PSMA3-AS1^{low} and PSMA3-AS1^{high} groups using the Kaplan–Meier method (log-rank test). The data are represented as the mean ± SD, n=3. ***P < 0.001.