Figure 2. Forced PSMA3-AS1 expression promotes ESCC cell proliferation, migration, and invasion in vitro. (A) PSMA3-AS1 expression in several ESCC cell lines was examined using RT-qPCR analysis. GAPDH was used as an internal loading control. (B) PSMA3-AS1 expression in ESCC KYSE150 and KYSE450 cells was modified by cDNA transfection. (C) and (D) Cancer cell proliferation was measured using CCK-8 (C) and clone formation assays (D). (E) Cancer cell migration was measured using wound healing assay. (F) Cancer cell invasion was measured using transwell assay. The data are represented as the mean ± SD, n=3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.