Figure 3. Decreased PSMA3-AS1 expression inhibits ESCC cell proliferation, migration, and invasion in vitro. (A) PSMA3-AS1 expression in ESCC EC9706 and EC109 cells was modified by shRNA transfection. (B) and (C) Cancer cell proliferation was measured using CCK-8 (B) and clone formation assays (C). (D) Cancer cell invasion was measured using transwell assay. (E) Cancer cell migration was measured by using wound healing assay. The data are represented as the mean ± SD, n=3. *p < 0.05, **p < 0.01, ***p < 0.001.