Research Paper Volume 12, Issue 2 pp 1843—1856

Long non-coding RNA PSMA3-AS1 promotes malignant phenotypes of esophageal cancer by modulating the miR-101/EZH2 axis as a ceRNA

Figure 4. PSMA3-AS1 functions as a ceRNA for miR-101 in ESCC cells. (A) Target sequences in PSMA3-AS1 predicted to bind to miR-101. (B) and (C) miR-101 expression after forced/decreased PSMA3-AS1 expression was detected in ESCC cells by RT-qPCR. (D) Wild-type or mutated PSMA3-AS1 was transfected into HEK-293T cells with miR-101 or a negative control. Luciferase activity was detected 48 h after transfection. (E) RT-qPCR showed PSMA3-AS1 levels in the streptavidin-captured fractions from KYSE-150 cell lysate after transfection with biotinylated miR-101 or negative control (NC). (F) RIP experiments were performed using an antibody against AGO2 in extracts from KYSE-150 cells. (G) AGO2 expression in ESCC KYSE150 and KYSE450 cells was modified by shRNA or cDNA transfection. (H) TPSMA3-AS1 expression after decreased AGO2 expression was detected in ESCC cells by RT-qPCR. The data are represented as the mean ± SD, n = 3. *p < 0.05; **p < 0.01.