Research Paper Volume 12, Issue 2 pp 1843—1856

Long non-coding RNA PSMA3-AS1 promotes malignant phenotypes of esophageal cancer by modulating the miR-101/EZH2 axis as a ceRNA

Figure 5. PSMA3-AS1 restores EZH2 expression by binding to miR-101. (A) Target sequences in the EZH2 3′-UTR predicted to bind to miR-101. (B) Wild-type or mutated 3′-UTRwas transfected into HEK-293T cells with miR-101 or a negative control. Luciferase activity was detected 48 h after transfection. (C) Wild-type or mutated 3′-UTRwas transfected into HEK-293T cells with miR-101 siRNA or a negative control. Luciferase activity was detected 48 h after transfection. (D) and (E) EZH2 expression was detected after PSMA3-AS1 expression upregulation in ESCC cells by RT-qPCR and western blotting. (F) and (G) EZH2 expression was detected after PSMA3-AS1 expression donwnregulation in ESCC cells by RT-qPCR and western blotting. (H) and (I) EZH2 expressions was detected after mutant PSMA3-AS1 expression upregulation in ESCC cells by RT-qPCR and western blotting. The data are represented as the mean ± SD, n = 3. *p < 0.05; **p < 0.01.