Figure 6. PSMA3-AS1-induced esophageal cancer cells progression in an EZH2 dependent manner. (A) EZH2 expression in ESCC KYSE150 and KYSE450 cells was modified by CRISPR/Cas9 gene editing. (B) and (C) Cancer cell proliferation was measured using CCK-8 (B) and clone formation assays (C). (D) Cancer cell invasion was measured by transwell assay. (E) Cancer cell migration was measured by wound healing assay. (F) Representative ESCC cases in the tissue microarray were analyzed by immunohistochemical staining for EZH2. (G). A positive correlation between PSMA3-AS1 and the number of EZH2-positive cells was observed in ESCC tissues (R2 = 0.2163; P < 0.001). The data are represented as the mean ± SD, n=3. *p < 0.05, **p < 0.01, ***p < 0.001.