Figure 6. The Rho/ROCK1 pathway participated in CNN1-induced breast cancer. (A) The half-time of ROCK1 transcript after CNN1 overexpression was decreased using half-time assay. The MCF-7 and MDA-MB-231 cells after transfection with CNN1 overexpression were treated with Act D (8 μg/ml) for 0 h, 2 h, 4 h, 6 h, and 8 h. The ROCK1 mRNA remaining was detected by qRT-PCR. (B) The protein expressions of Rho and ROCK1 was decreased after CNN1 overexpression. The western blot assay was used to measure the protein expression after CNN1 overexpression for 24 h. Control, the cells were cultured without any treatment. NC, the cells were treated with the negative control. CNN1 OE, the cells were treated with CNN1 overexpression. *P<0.05 vs. Control and **P<0.001 vs. Control.