Research Paper Volume 12, Issue 2 pp 1867—1887

miR-106b-5p contributes to the lung metastasis of breast cancer via targeting CNN1 and regulating Rho/ROCK1 pathway

Figure 8. miR-106b-5p mimic alleviated the role of CNN1 overexpression induced suppression of cell proliferation and invasion. (A) Co-transfection of miR-106b-5p mimic and CNN1 overexpression led to the downregulation of CNN1 and upregulation of miR-106b-5p both in MCF-7 and MDA-MB-231 cells. (B) Co-transfection of miR-106b-5p mimic and CNN1 overexpression resulted in the decrease of the protein level of CNN1 compared with transfection of CNN1 overexpression. OE, the cells were transfected with CNN1 overexpression. OE+mimic, the cells were co-transfected with miR-106b-5p mimic and CNN1 overexpression. *P<0.05 vs. Control and **P<0.001 vs. Control. (C) and (D) The co-transfection of miR-106b-5p mimic and CNN1 overexpression alleviated the inhibition of cell proliferation and invasion caused by CNN1 overexpression. The CCK8 assay and transwell assay respectively revealed the changes of cell proliferation and invasion. CNN1 OE, the cells were transfected with CNN1 overexpression. CNN1 OE+mimic, the cells were co-transfected with miR-106b-5p mimic and CNN1 overexpression. *P<0.05 vs. Control and **P<0.001 vs. Control. #P<0.05 vs. CNN1 OE. #P<0.001 vs. CNN1 OE.