Figure 8. The increased expression of AURKA and CDC20 along with the progression of HCC from stage 1 to 4. (A and B) The expression of AURKA and CDC20 in 20 nontumor samples and 66 HCC tissues from patients with HCC who had been clinically divided into stage 1 (n = 20), stage 2 (n = 20), stage 3 (n = 20), and stage 4 (n = 6) by Barcelona Clinic Liver Cancer staging system. (C) Analysis of AURKA and CDC20 protein level in representative 2 normal liver tissues and 12 HCC tissues from patients in HCC stage 1 to 4 by western blots. (D) Representative images (200×) from immunohistochemistry staining analysis of AURKA and CDC20 expression in normal liver tissues and different stages of HCC tissues. (E) Quantification analyses of H-score of AURKA and CDC20 staining in normal liver tissues and different stages of HCC tissues. *p<0.05, **p<0.01, ***p<0.001.