Figure 1. The GB cellular models show different levels of CXCL8 and CXCR1/2. ELISA assay was used to quantify the amount of CXCL8 secreted in the supernatant media from GB primary cell culture and U-87MG cells (A). Data are means ± SEM of three different biological replicates (n=3). (B) Representative cytofluorimetric analysis for CXCR1 and CXCR2 protein levels in GB primary cell culture and U-87MG cell line. Cytofluorimetric profile images are representative one. Cytofluorimetric analysis were performed in permeabilized or not permeabilized cells. tCXCR1/2: total protein levels in permeabilized cellular samples; sCXCR1/2: surface protein levels in not permeabilized cellular samples.