Figure 4. DF2755A modulates the activity of the protein involved in cell motility and NF-κB p65 nuclear translocation. Representative western blotting and relative densitometry analysis for p-FAK (Tyr397)/Fak, p-cortactin (Tyr466)/cortactin, PI3K, p-Akt (Thr308)/Akt in GB primary cell culture (A) and U-87MG cells (B) untreated and treated with DF2755A. Western blotting and relative densitometry analysis for cytoplasmic (normalized on the β-Actin) and nuclear (normalized on Lamin B1) NF-κB p65 and its cytoplasmic inhibitor IκBβ in GB primary cell culture (C) and U-87MG cells (D) untreated and treated with DF2755A. Data are mean ± SEM of three different biological replicates (n=3). Statistical analysis was performed by the unpaired Student's t-test (with Welch’s correction). *, p< 0.05; **, p< 0.01; ***, p< 0.001, Ctr vs DF2755A were considered statistically significant. Ctr: Control, DF: DF2755A 0.1 μM. R.U.: relative units.