Research Paper Volume 12, Issue 2 pp 1928—1951

Autocrine CXCL8-dependent invasiveness triggers modulation of actin cytoskeletal network and cell dynamics

Figure 5. Cytoskeletal and microtubules dynamics are adversely affected by DF2755A in GB primary cell culture. (A) Representative western blotting and relative densitometry analysis for RhoA, Cdc42, Acetylated α-tubulin (Lys40)/α-tubulin. Data are mean ± SEM of three different biological replicates (n=3). Statistical analysis was performed by the unpaired Student's t-test (with Welch’s correction). **, p< 0.01, Ctr vs DF2755A were considered statistically significant. Ctr: Control, DF: DF2755A 0.1 μM. R.U.: relative units. (B) Immunolocalization of RhoA and microfilament decoration by phalloidin-staining in control and DF2755A treated GB primary cell cultures. White arrowheads indicate filopodia, their retraction is clear in magnified images of DF2755A treated cells. Ctr: Control, DF: DF2755A 0.1 μM. Scale bar = 50 μm.