Figure 5. Cytoskeletal and microtubules dynamics are adversely affected by DF2755A in GB primary cell culture. (A) Representative western blotting and relative densitometry analysis for RhoA, Cdc42, Acetylated α-tubulin (Lys40)/α-tubulin. Data are mean ± SEM of three different biological replicates (n=3). Statistical analysis was performed by the unpaired Student's t-test (with Welch’s correction). **, p< 0.01, Ctr vs DF2755A were considered statistically significant. Ctr: Control, DF: DF2755A 0.1 μM. R.U.: relative units. (B) Immunolocalization of RhoA and microfilament decoration by phalloidin-staining in control and DF2755A treated GB primary cell cultures. White arrowheads indicate filopodia, their retraction is clear in magnified images of DF2755A treated cells. Ctr: Control, DF: DF2755A 0.1 μM. Scale bar = 50 μm.