Research Paper Volume 12, Issue 2 pp 1965—1986

Anesthesia and surgery induce age-dependent changes in behaviors and microbiota

Figure 6. Lactobacillus attenuate the anesthesia/surgery-induced changes in the 18 months old mice. Interaction of anesthesia/surgery and the treatment of Lactobacillus on the buried food test (A), Y maze test (duration in novel arm, B), Y maze test (entries in novel arm, C), Y maze test (number of arm visit,D), open field test (total distance, E), open field test (freezing time, F), open field test (latency to center, G), open field test (Time spent in the center, H) in the 18 months old mice. The comparison of the qualitative (I) or quantitative (J) changes of the postoperative delirium-like behavior between the 18 months old mice received the anesthesia/surgery plus saline and the 18 months old mice received the anesthesia/surgery plus lactobacillus. The treatment of Lactobacillus attenuated the anesthesia/surgery-induced increase in the composite Z score. (K) The comparison of the brain mitochondrial function between the 18 months old mice received the anesthesia/surgery plus saline and the 18 months old mice received the anesthesia/surgery plus lactobacillus. The treatment of Lactobacillus attenuated the anesthesia/surgery-induced mitochondrial dysfunction. n = 10 - 12 in each group of the behavioral studies, and n = 6 in each group of the mitochondrial function studies. Friedman test was used to analyze the data in the (AC, F and H). Two-way ANOVA and post-hoc Bonferroni comparison was used to analyze the data in the (D, E, G and J). * = P < 0.05.