Figure 2. Effects of the MAPK and PI3K pathway inhibitors on IGFBP3 releasing from MESCs in conditioned media (CM). Cells were treated with 200 μM H2O2 for 1 h, or cells pretreated with one of specific inhibitors (10 μM U0126, 5 μM SB203580, 20 μM LY294002, 10 μM SP600125,) for 30 min were exposed to 200 μM H2O2 for 1 h, and then incubated with each of inhibitors for the indicated time; Ctr – untreated cells. The IGFBP3 concentration in CM was quantified by the ELISA. Mean values ± SD of three independent experiments are shown; p<0.01 for H2O2 (except 2 d) and each of inhibitors at the indicated time periods compared with Ctr (except U0126, 2 d and 4 d). Also, p<0.01 for LY at each time point, as well as for SP and SB at 6 day compared with H2O2 at the same time point.