Figure 1. H2O2 induced HLEC injury. HLEC SRA01/04 cells were treated with 400 μM H2O2 for 1 h, and non-treated cells were acted as control. (A, B) Cell viability was measured by CCK-8 assay. (C, D) Expression of p53, cyclinD1 and CDK4 was testified by Western blot analysis. (E, F) Percentage of apoptotic cells was quantified by flow cytometry assay and TUNEL assay. (G) Expression of proteins related to apoptosis was detected by Western blot analysis. (H) γH2AX staining for detection of DNA levels. Data are shown as the mean ± SD of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.