Research Paper Volume 12, Issue 8 pp 6543—6557

Long non-coding RNA ANRIL alleviates H2O2-induced injury by up-regulating microRNA-21 in human lens epithelial cells

Figure 2. LncRNA ANRIL attenuated H2O2-induced HLEC injury. HLEC SRA01/04 cells were transfected with pcDNA3.1 or pc-ANRIL, and untransfected cells were acted as control. (A) Expression of lncRNA ANRIL was determined by RT-qPCR. Transfected or untransfected SRA01/04 cells were treated with 400 μM H2O2 for 1 h, and non-treated cells were acted as control. (B) Cell viability was measured by CCK-8 assay. (C, D) Expression of p53, cyclinD1 and CDK4 was testified by Western blot analysis. (E, F) Percentage of apoptotic cells was quantified by flow cytometry assay. (G) Expression of proteins related to apoptosis and (I) p14, p15 and p19 were detected by Western blot analysis. (H) γH2AX staining for detection of DNA levels. Data are shown as the mean ± SD of three independent experiments. *, P < 0.05; ***, P < 0.001.