Research Paper Volume 12, Issue 8 pp 6543—6557

Long non-coding RNA ANRIL alleviates H2O2-induced injury by up-regulating microRNA-21 in human lens epithelial cells

Figure 4. miR-21 inhibition reversed the effects of lncRNA ANRIL on H2O2-treated HLECs. HLEC SRA01/04 cells were transfected with inhibitor-NC or miR-21 inhibitor, and untransfected cells were acted as control. (A) Expression of miR-21 was determined by RT-qPCR. Cells co-transfected with pcDNA3.1 (pc-ANRIL) and miR-21 inhibitor (inhibitor-NC) or untransfected cells were treated with 400 μM H2O2, and non-treated cells were acted as control. (B) Cell viability was measured by CCK-8 assay. (C, D) Expression of p53, cyclinD1 and CDK4 was testified by Western blot analysis. (E, F) Percentage of apoptotic cells was quantified by flow cytometry assay. (G) Expression of proteins related to apoptosis was detected by Western blot analysis. (H) γH2AX staining for detection of DNA levels. Data are shown as the mean ± SD of three independent experiments. *, P < 0.05; ***, P < 0.001.