Research Paper Volume 12, Issue 8 pp 6543—6557

Long non-coding RNA ANRIL alleviates H2O2-induced injury by up-regulating microRNA-21 in human lens epithelial cells

Figure 6. LncRNA ANRIL activated AMPK and β-catenin in H2O2-treated HLECs via up-regulation of miR-21. HLEC SRA01/04 cells co-transfected with pcDNA3.1 (pc-ANRIL) and miR-21 inhibitor (inhibitor-NC) or untransfected cells were treated with 400 μM H2O2 for 1 h, and non-treated cells were acted as control. Phosphorylation of AMPK (A, B) and expression of β-catenin (C, D) were testified by Western blot analysis. (E) ROS production was tested through ROS assay; (F) cell viability was tested by CCK-8. SRA01/04 cells were transfected with pcDNA3.1 or pc-ANRIL and treated with Compound C or XAV939, and untreated cells were acted as control. (G) Expression of lncRNA ANRIL and miR-21 were determined by RT-qPCR. Data are shown as the mean ± SD of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.