Research Paper Volume 12, Issue 7 pp 5625—5639

LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p

Figure 1. Expression pattern of lncRNA 2310043L19Rik. (A) Real-time PCR analysis of lncRNA 2310043L19Rik expression in 6 types of mouse tissues. Mean values ± SEM, n=3, *P< 0.05, **P< 0.01. Mice were 8-week old. (B) Real-time PCR analysis of MyoG expression in C2C12 during 4 days of differentiation. (C) Real-time PCR analysis of lncRNA 2310043L19Rik expression in C2C12 during 4 days of differentiation. Mean ±SEM, n=3. (D) Semi-quantitative RT-PCR analysis of mRNA expression of Atrogin-1, the muscle atrophy marker gene marker, in the tibialis anterior muscle of young (8 weeks) and aged (16 months). (E) Real-time PCR analysis of lncRNA 2310043L19Rik expression in young mouse (8 weeks) and aged (16 months) mice in the tibialis anterior muscle. Mean values ± SEM, n=3, *P< 0.05, **P< 0.01, ***P< 0.001.